Immunoprecipitation is a technique used to selectively purify an antigen of interest present in a complex mixture. This is accomplished through the use of a specific antibody and a solid phase matrix to bring the antigen-antibody complex out of solution. This technique is often used to study the presence or absence of an antigen, the quantity of an antigen at a given time point, relative molecular weights of proteins, protein-protein interactions or post-translational modifications, or the rate of synthesis or degradation of antigens.

Antigens that have been recovered by immunoprecipitation can be utilized in a number of downstream applications, including functional assays and detection. One method of visualization of the purified antigen is Western Blotting. This method includes electrophoresis of the protein on a SDS PAGE gel followed by transfer to membrane and Western detection. KPL offers reagents for protein purification, immunoprecipitation, and protein analysis. 

The ProteoSOL^TM Tissue Extraction System lets you solubilize proteins in FFPE tissue samples for relative quantitative protein expression analysis, thereby enabling immunodetection of disease biomarkers.